Oxygen permeability of thylakoid membranes: electron paramagnetic resonance spin labeling study

Electron Spin Resonance Studies of Ionic Permeability Properties of Thylakoid Membranes of Beta vulgaris and Avicennia germinans.

Measurement of intrathylakoid aqueous volumes by electron spin resonance spectroscopy was used to study ionic permeability properties of thylakoid membranes isolated from Beta vulgaris L. and Avicennia germinans L. The thylakoids behaved as perfect osmometers in the presence of sorbitol and betaine. Thylakoids exposed to hypertonic solutions of NaCl and KCl shrank and subsequently swelled, requ...

electron-paramagnetic-resonance studies using oxygen-17

Studies of the effect of substitution with 170 on the e.p.r. spectra at 9 and 35 GHz of Mo(V) in the phosphate complex of sulphite oxidase are reported. Substitution of '7O-enriched water for normal water, for samples of the enzymes reduced by sulphite in the presence of normal phosphate, produced no detectable effect on the e.p.r. signal. If phosphate substituted with 170 was used, coupling du...

Sequence of oxygen binding by hemoglobin ( hemoglobin subunits / spin - labeling / electron paramagnetic resonance / heme - heme interactions ) TOSHio ASAKURA AND PUI

A nitroxide spin-label probe was attached directly to a propionic acid group of heme in either the a or the P chain of hemoglobin. The electron paramagnetic resonance (EPR) spectrum of the spin label is altered by the spin-state change of the heme iron to which the spin label is attached. These hybrid hemoglobins showed normal optical and functional properties, indicating that the attachment of...

Structure of the inhibitory region of troponin by site directed spin labeling electron paramagnetic resonance.

Site-directed spin labeling EPR (SDSL-EPR) was used to determine the structure of the inhibitory region of TnI in the intact cardiac troponin ternary complex. Maeda and collaborators have modeled the inhibitory region of TnI (skeletal 96-112: the structural motif that communicates the Ca(2+) signal to actin) as a kinked alpha-helix [Vassylyev, D., Takeda, S., Wakatsuki, S., Maeda, K. & Maeda, Y...

Nitric Oxide in Membranes and Aqueous Media: Spin Trapping and Interaction with Spin Labels. an Electron Paramagnetic Resonance Spectroscopic Study